The role of religion in development is often neglected, whether this refers to the faith of intended beneficiaries, provides staff and volunteers with a motivation for involvement in development practice or influences the design and implementation of projects. This paper examines how Islam provides guidance for development practice, with a focus on addressing HIV/AIDS, using a South African case study. The case study highlights important principles on which two Muslim organisations (Islamic Careline and the Muslim AIDS Programme) base their operational methodologies.